Autoradiography principle ppt

Title: Autoradiography


1
AUTORADIOGRAPHY
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Radioisotopes

• Isotopes(which have same chemical but different
  physical properties) having radioactivity.

• Natural occurrence is rare because of its
  instability.
• They emit different radiations such as a,ß and ?.

3
Detection of disintegration

• Electrical
• Eg GM counter, Ionisation
  counter, Gas flow counter
• Scintillation
• Eg scintillation counter
• Autoradiography

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What is autoradiography?

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Principle
Radiation will hit silver grains in emulsion and
expose them
Expose to film or emulsion
Isotope will emit radiation (usually beta)
Incubate tissue with radioactive ligand
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HISTORY

• 1867 Uranium salts responsible for blackening
• of photographic film(Niepce de
  st.victor)
• 1896 K2UO2(SO4)2emits radiation affecting a
• photographic film(Becquerel)
• 1924 First autoradiogram distribution of Po
  in
• biological material
• 1943 Autoradiographic demonstratio